EasyStep Rat NPT (Neopterin) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPT antigen, and the Rat NPT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat NPT to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat NPT in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 0.469-30 nmol/L. Sensitivity: 0.4 nmol/L. Assay Type: Competitive Inhibition. Standard: 30 nmol/L. Sample Type: sSerum, plasma. Assay duration: 1.5h.