EasyStep Human Lpa(Lipoprotein a) ELISA Kit

Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330 Assay Type: Sandwich. Sensitivity: 0.38 ng/mL. Standard: 50 ng/mL. Detection range: 0.78-50 ng/mL. Sample type: Serum, plasma. Assay length: 1.5h. Research Field: Metabolic pathway,Cardiovascular biology,Hepatology,Gastroenterology,Nutrition metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Lpa, and the Human Lpa standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Lpa. After TMB substrate solution is added, only those wells that contain Human Lpa and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Lpa in the samples is then determined by comparing the OD of the samples to the standard curve.