EasyStep Human aFP (Alpha-Fetoprotein) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human aFP, and the Human aFP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human aFP. After TMB substrate solution is added, only those wells that contain Human aFP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aFP in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 1.56-100 ng/mL. Sensitivity: 0.175 ng/mL. Assay Type: Sandwich. Standard: 100 ng/mL. Sample Type: sSerum, plasma. Assay duration: 1.5h. Protein Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties [The Uniprot Consortium]