DNA Methylation ELISA Kit

DNA methylation is an important epigenetic process regulating gene expression. Methylation occurs on carbon 5 of 2'-deoxy-cytidine yielding the modified base 5-methyl-2'-deoxy cytidine. The methylation pattern of cells is tightly regulated during development with the methylation profile being transmitted from parent to daughter cells during cell division. Methylation results in long-term silencing of genes, while unmethylated regions of DNA can be actively transcribed. Global changes in methylation can be quantified by measuring plasma or urinary levels of 5-methyl-2'-deoxy cytidine. These changes in methylation can provide valuable information about cancer status of an individual. For example, patients with leukemia excrete significantly elevated levels of 5-methyl-2'-deoxy cytidine compared to healthy individuals. Global methylation within tissues can be measured in a similar manner, allowing study of tissue-specific changes that occur as a result of differentiation, aging, or carcinogenesis. Cayman's DNA Methylation ELISA Kit is a competitive assay that can be used for the quantification of 5-methyl-2'-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices. The ELISA typically displays IC50 (50% B/B0) and IC80 (80% B/B0) values of approximately 12 and 3 ng/ml, respectively.Synonyms: 5-Methyl-2'-deoxycytidine EIA Kit. Assay Range: 1.17-150 ng/ml. Sensitivity: 80% B/B0: ~3 ng/ml. Mid-point: 50% B/B0: ~8-15 ng/ml. Cross Reactivity: (5-Methyl-2'-deoxycytidine) 100%, (5-Methylcytidine) 20%, (2-Deoxycytidine) 0.1%, (Cytidine) 0.1%, (Thymidine) <0.01%. Origin: Animal/Goat, Animal/Bovine, Animal/Eel, Animal/Mouse.