CLPB (Caseinolytic Peptidase B Protein Homolog) BioAssay(TM) ELISA Kit (Human) (HSP78)

Sample Type:, Serum, plasma and other biological fluids. Intended Use: Sandwich ELISA, Double Antibody, Sensitivity: <0.469ng/ml, Range: 0.781-50ng/ml, Specificity: Human, Test Principle: This BioAssay(TM) kit was based on sandwich enzyme-linked immune-sorbent assay technology. CLPB was pre-coated onto 96-well plates. And the biotin conjugated CLPB was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of can be calculated. Kit Components: Micro ELISA Plate (Dismountable), 8x12, Lyophilized Standard, 2xvial, Sample/Standard dilution buffer, 20ml, Biotin-detection antibody (Concentrated), 120ul, Antibody dilution buffer, 10ml, HRP-Streptavidin Conjugate (SABC), 120ul, SABC dilution buffer, 10ml, TMB substrate, 10ml, Stop solution, 10ml, Wash buffer (25X), 30ml, Plate Sealer, 5xpieces, Product Description, 1xcopy, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.