CD163 BioAssay(TM) ELISA Kit, Human

CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc. Uniprot ID: Q86VB7, Entrez Gene ID: 9332, Intended Use: CD163 BioAssay(TM) ELISA Kit is a sandwich ELISA for quantitative detection of human CD163 in cell culture supernates, serum and plasma (heparin). Range: 1.56-100ng/ml, Sensitivity: <150 pg/ml , Specificity: Natural and recombinant human CD163, Crossreactivity: No detectable crossreactivity with other relevant proteins, Intra-Assay CV(%): 4.2, 4.5, 5.3, Inter-Assay CV(%): 6.7, 5.4, 5.6, Kit Components: 143759A: Microtiter Strips, 1x96 wells, *143759B: CD163 Standard, 2x1 vial, 143759C: Anti-human CD163 (Biotin), 1x130ul, USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *143759B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. , 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS., 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes., 5. Add TMB stop solution and read.