Carbonic Anhydrase II (CA2) BioAssay(TM) ELISA Kit (Human)

Carbonic Anhydrase II (CA2) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CA2 in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Detection Range: 3.12-200ng/ml, Sensitivity: 1.34ng/ml, Intra-Assay: CV?10%, Inter-Assay: CV?12%, Kit Components: *023901A: Microtiter Plate, 1x96wells, Pre-coated, ready to use, *023901B: Standard, 2x1vial , 023901C: Standard Diluent, 1x20ml, *023901D: Detection Reagent A, 1x120ul , *023901E: Detection Reagent B, 1x120ul , 023901F: Assay Diluent A, 2x, 1x6ml, 023901G: Assay Diluent B, 2x, 1x6ml, 023901H: TMB Substrate, 1x9ml, *023901J: Positive Control, 18.92ng/ml, 1x1vial, 023901K: Stop Solution, 1x6ml, 023901L: Wash Buffer, 30X, 1x20ml , Precaution: The Stop Solution (023594K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023901A, *023901B, *023901D, *023901E and 023901J at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, , 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37°C. , 4. Aspirate and wash 3 times. , 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. , 5. Aspirate and wash 5 times., 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C. , 7. Add 50ul Stop Solution. Read at 450nm immediately.