BMP-9, Mouse, BioAssay(TM) DuoSet ELISA Development Kit (GDF2, GDF-2)

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype. Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks. Sample Type: Cell culture supernates, Intended Use: This BioAssay(TM) DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Bone Morphogenetic Protein 9 (BMP-9). DuoSets are designed for the analysis of cell culture supernates. Specificity: Recognizes natural and recombinant mouse Bone Morphogenetic Protein 9 (BMP-9). This mouse BMP-9 DuoSet also recognizes recombinant human BMP-9. Kit Components: Sheep anti-mouse BMP-9 capture antibody, 1x vial, Biotinylated sheep anti-mouse BMP-9 detection antibody, 1x vial, Recombinant sheep anti-mouse BMP-9 standard, 3x vials, Streptavidin-HRP, 1x vial, Storage and Stability: Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.