Amphiregulin (AR) BioAssay(TM) ELISA Kit (Human)

Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA), saliva and human milk, Intended Use: For quantitative detection of human Amphiregulin in cell culture supernates, serum, plasma (heparin, EDTA), saliva and human milk. Sensitivity: <10pg/ml, Range: 15.6-1000pg/ml, Specificity: Natural and recombinant human Amphiregulin, Test Principle: This human Amphiregulin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Amphiregulin has been precoated onto 96-well plates. Standards (E.coli) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Amphiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Amphiregulin amount of sample captured in plate. Kit Components: 96-well plate precoated with anti- human Amphiregulin antibody, 1, Lyophilized recombinant human Amphiregulin standard, 10ng/tube×2, Biotinylated anti- human Amphiregulin antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.