AAV8 Chemiluminescence ELISA Titration Kit

AAV8 Chemiluminescence ELISA Titration Kit is a sandwich ELISA (Enzyme-linked immunosorbent assay) designed to detect and quantify AAV8 (adeno-associated virus type 8) non-denatured capsids. It provides accurate, specific, and sensitive assessment of the number of AAV8 capsids (full and empty) present in purified AAV8 samples. This kit contains all the materials necessary for 100 reactions, including high sensitivity ELISA ECL reagents. This kit has a linear range between 7.8x10^7 - 1x10^10 capsids/ml. Note: The capture and detection antibodies detect a conformational epitope not present on unassembled capsid proteins. Figure 1. AAV8 Chemiluminescence ELISA Titration Kit schematic. Coat the plate with the Anti-AAV8 Coating Antibody. Prepare a serial dilution of the reference virion and of your samples of interest, add to the coated plate, wash, and add the Anti-AAV8 Detection Antibody followed by anti- HRP-labeled secondary antibody. The chemiluminescence signal is directly correlated with the number of AAV8 capsids present. Recombinant adeno-associated virus (rAAV) is a widely used vector in gene therapy applications. rAAVs exist in the nucleus as episomes and are unable to integrate into the cellular genome. While this brings safety benefits, as there is no risk of random integration and activation of oncogenes, the cell division rate dictates the half-life for transgene expression. Their application is thus particularly useful for the delivery of transgenes to post-mitotic tissues like the brain, retinal, liver, skeletal muscle, and the heart. The payload carried by AAVs is somewhat smaller than other viral tools used for transduction, usually smaller than 5 kb. There are several serotypes, each one with a specific tissue tropism. During production, AAV titers can be measured as a physical titer, infectious titer, or ratio of full to empty capsids. Accurately determining the titer is essential not only for setting clinical doses but also as a foundational step in various analytical assays for characterizing AAV products. The use of an ELISA-based assay to measure AAV8 particles allows for better inter- and intra-laboratory consistency, compared to the use of primers in qPCR. This is a well-suited kit for adoption in gene therapy research and can be further automated for high-throughput applications.