TSLPR:TSLP [Biotinylated] Small Molecule Inhibitor Screening Chemiluminescence Assay Kit

The TSLPR:TSLP [Biotinylated] Small Molecule Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling small molecules that block the binding between TSLPR (thymic stromal lymphopoietin receptor) and TSLP. This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled TSLP (amino acids 29-159), human TSLPR (amino acids 25-231), streptavidin-HRP, and assay buffer for 100 reactions. A 96-well plate is coated with TSLPR protein. After coating and blocking, TSLP is added in an optimized assay buffer. Unbound TSLP is washed away, and the plate is incubated with streptavidin-HRP. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of TSLP binding to TSLPR.