Transcreener(R) UDP2 TR-FRET Assay

The Transcreener(R) UDP2 TR-FRET Red Assay is a competitive immunoassay for UDP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for UDP, the assay can be used with any enzyme that generates UDP from a UDP-sugar donor. Examples include glycosyltransferase, galactosyltransferase, glucuronyltransferase, N-acetylglucosamyltransferase, N-acetylgalactosyltransferase, xylosyltransferase, and glycogen, cellulose, lactose, and hyaluronan synthases. ¶The Transcreener assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. ¶The Transcreener(R) UDP2 TR-FRET Red Assay provides the following benefits:  Accommodates UDP-sugar donor concentrations ranging from 1 µM to 1,000 µM.  Excellent data quality (Z' > 0.7) at low substrate conversion (typically 10-30%).  Overcomes the need for time-consuming, one-off assay development for individual members within an enzyme family by using a single set of assay reagents that detect an invariant product.  Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.  Far-red tracer further minimizes interference from fluorescent compounds and light scattering.