Transcreener(R) UDP2 FP Assay

The Transcreener(R) UDP2 FP Assay is a universal biochemical HTS assay for enzymes that produce UDP, including glycosyltransferase, galactosyltransferase, glucuronyltransferase, N-acetylglucosamyltransferase, N-acetlygalactosyltransferase, xylosyltransferase, and glycogen, cellulose, lactose and hyaluronan synthases. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener UDP2 Antibody. The assay is a simple single step mix-and-read format enabling the use of unmodified native substrate concentrations of 1 - 1000 µM. The assay provides excellent signal at low substrate conversion, with a Z' >0.7 and >100 millipolarization shift (mP) under normal reaction conditions.