Transcreener(R) OAADPr SIRT TR-FRET Assay

The Transcreener OAADPr SIRT TR-FRET Assay is a biochemical HTS assay for measuring the production 2'-O-Acetyl-ADP-ribose (OAADPr) by sirtuins (SIRT), NAD-dependent deacetylases. The assay relies on a Coupling Enzyme (CE) to convert OAADPr into AMP, which is then detected using a far-red, competitive, time-resolved Förster-resonance-energy-transfer (TR-FRET) assay. As an example, the Transcreener OAADPr SIRT TR-FRET assay can be used to detect the activity of human SIRT1 and SIRT2 using acetylated peptides as substrates.