Transcreener(R) OAADPr SIRT FP Assay

The Transcreener OAADPr SIRT FP Assay is a biochemical HTS assay for measuring the production of 2'-O-acetyl-ADP-ribose (OAADPr) by sirtuins (SIRT), NAD-dependent deacetylases. The assay relies on a Coupling Enzyme (CE) to convert OAADPr into AMP, which is then detected using a far-red, competitive fluorescence polarization (FP) assay. As an example, the Transcreener OAADPr SIRT FP assay can be used to detect the activity of human SIRT1 and SIRT2 using acetylated peptides as substrates.