Transcreener(R) cGAMP cGAS TR-FRET Assay

The Transcreener(R) cGAMP cGAS TR-FRET Assay is a far-red, competitive fluorescence polarization (FP) assay. The Transcreener(R) assay is designed specifically for high throughput screening (HTS), with a single addition, mix-and-read format. Because the antibody is highly selective for cGAMP, the assay can be used to measure activity of the cyclic GMP-AMP synthase (cGAS) enzyme which converts ATP and GTP, to cGAMP. cGAS is a recently discovered enzyme that acts as a foreign DNA sensor that induces an immune response via activation of the stimulator of interferon genes (STING) receptor. By directly measuring cGAMP with a highly selective antibody, it is possible to assay the activity of cGAS while screening large compound libraries for inhibitors. The Transcreener(R) cGAMP cGAS TR-FRET Assay provides the following benefits:  A simple single addition cGAS activity assay capable of HTS.  Excellent data quality (Z' > 0.7) and signal (>85 mP polarization shift) at cGAMP ranges between 0.5 µM and 5 µM.  Far-red tracer further minimizes interference from fluorescent compounds and light scattering.