Transcreener(R) 2-5A OAS FP Activity Assay

The Transcreener(R) OAS FP Assay is a far-red, competitive fluorescence polarization (FP) assay. The assay is designed to be used with the 2'-5'-oligoadenylate synthase (OAS) family of enzymes, such as OAS1, OAS2, and OAS3), that produce the product 2-5A. The Transcreener OAS FP Assay is a simple biochemical assay for measuring OAS activity based on the Transcreener AMP2 Assay. The assay uses a coupling enzyme to convert 2-5A into AMP in the presence of ATP and dsRNA. The Transcreener assay is designed specifically for high throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The Transcreener OAS FP Assay provides the following benefits:  A simple single addition OAS activity assay capable of HTS.  Excellent data quality (Z' > 0.7) and signal (>100 mP polarization shift) for determining OAS activity by measuring 2-5A production.  Far-red tracer further minimizes interference from fluorescent compounds and light scattering.