Sialidase Fluorogenic Assay Kit

The Sialidase Fluorogenic Assay Kit is designed to measure Sialidase NANH (N-acylneuraminate glycohydrolase) activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinant Salmonella typhimurium sialidase, Sialidase substrate, Sialidase assay buffer, and Stop Solution for 100 enzyme reactions. Sialidase inhibitor NCGC00063279 (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid) is also included as a control. The Sialidase fluorogenic substrate is incubated with Sialidase. Hydrolyzation of the Sialidase substrate releases a fluorophore (lambdaexc/lambdaem = 365 nm/445 nm). The increase in fluorescence at lambda=445 nm is directly proportional to Sialidase activity.