PRMT3 chemiluminescent assay kit

The PRMT3 Direct Activity Assay kit is designed to measure PRMT3 activity using purified PRMT3 or PRMT3 extracts. The PRMT3 Direct Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with specific substrate, the antibody against methylated substrate, the secondary Europium-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified PRMT3 enzyme for 100 enzyme reactions. The key to the PRMT3 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.