PARP14 Chemiluminescent Assay Kit

The PARP14 Chemiluminescent Activity Assay Kit is designed to measure PARP14 activity for screening and profiling applications. PARP14 catalyzes NAD-dependent ADP-ribosylation, and PARP14 overexpression in myeloma cells is associated with disease progression and poor survival rates. The PARP14 assay kit comes in a convenient 96-well format, with purified PARP14 enzyme, histone mixture, and PARP assay buffer for 32 enzyme reactions. The key to the PARP14 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP14 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP14 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.