Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit

Detection instrument: Microplate reader. Detection principle: Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The specific inhibitor rotenone can inhibit the enzyme, and the total enzyme activity minus the enzyme activity after inhibitor inhibition can reflect its specific enzyme activity. Sample type: animal tissue. Research area: Enzymes,Tricarboxylic Acid(TCA) Cycle. Assay time: 15 min. Sensitivity: 15.58 U/L. Detection range: 15.58-224.2 U/L. Recovery rate: 102. Inter CV: 8.4. Intra CV: 0.8