JARID1C Homogeneous Assay Kit

The JARID1C Homogeneous Assay Kit is designed to measure activity of the JARID1C for screening and profiling applications. JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3. The JARID1C Homogeneous Assay Kit comes in a convenient AlphaLISA format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1C for 384 enzyme reactions. The key to the JARID1C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JARID1C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.