DCAF1 Binding Assay Kit

The DCAF1 Binding Assay Kit is a fluorescence polarization (FP), homogeneous, 96-well assay kit designed for the screening and profiling of molecules that inhibit the binding of the DCAF1 (DDB1 and CUL4 Associated Factor 1) Complex to a high affinity DCAF1 fluorescent probe. The kit contains enough purified recombinant DCAF1/Rbx1 (M5I)/DDB1/CUL4A Complex, fluorescent probe and assay buffer for 100 reactions. The assay uses a DCAF1 fluorescent probe. This is a very small molecule that rotates fast (low FP). This probe is incubated with the DCAF1 complex leading to the formation of a large complex, with restricted movement (high FP). FP is proportional to binding of DCAF1 to the fluorescent probe. This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required parts to read the FP signal. For more information FP technology, visit our Tech Note: FP, assay principles and applications.