Cytochrome P450 Reductase (CPR) Activity Assay Kit

Detection principle: This kit uses the electron transfer characteristics of CPR to catalyze the substrate to generate chromophore, which has a maximum absorption at 450 nm. The activity of CPR can be calculated by measureing the OD value at 450 nm. Performance characteristics: Sample type: tissue, cell. Sensitivity: 9.11 U/L. Detection range: 9.11-550 U/L. Detection method: Colorimetric method. Assay type: Enzyme Activity. Assay time: 45 min. Precision: Average inter-assay CV: 3.2-3.9%, Average intra-assay CV: 2.8-3.8%. Other reagents required: Normal Saline (0.9% NaCl). Storage: -20?. Valid period: 12 months. Protein function: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. [The UniProt Consortium]