Citrate Synthase(CS) Activity Assay Kit

Detection instrument: Microplate reader. Detection principle: Citrate synthetase (CS) catalyzes acetyl-CoA and oxaloacetate to produce limonyl-CoA, which can further produce CoA. The yellow product generated by this reaction has a characteristic absorption peak at 412 nm. The activity of CS can be calculated by measuring the change of absorbance value at 412 nm. Sample type: animal/plant tissue and cell. Research area: Enzymes. Assay time: 30 min. Sensitivity: 2.09 U/L. Detection range: 2.09-110 U/L. Recovery rate: 104. Inter CV: 7.9. Intra CV: 5