Chemiluminescent PARP11 Assay kit

The PARP11 Chemiluminescent Activity Assay kit is designed to measure PARP11 activity for screening and profiling Applications. PARP11 is known to catalyze NAD-dependent ribosylation. The PARP11 assay kit comes in a convenient 96-well format, with purified PARP11 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions. The key to the PARP11 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP11 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP11 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Components: PARP11, 60 µg, 5x histone mixture, 1 ml, 20x PARP11 assay mixture containing biotinylated substrate, 250 µl, 10x PARP assay buffer, 1mL, Blocking buffer, 25 ml, Streptavidin-HRP, 100 µl, HRP chemiluminescent substrate, 2 components, 6 ml each, 96-well module. Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.