Chemi-Verse(TM) HPK1 (MAP4K1) Kinase Assay Kit

The Chemi-Verse(TM) HPK1 Kinase Assay Kit is designed to measure HPK1 (Hematopoietic progenitor kinase 1) serine/threonine kinase activity for screening and profiling applications using ADP-Glo(TM) as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified HPK1 (amino acids 1-346, kinase domain), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions. Note: MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.