CD155:TIGIT [Biotinylated] Inhibitor Screening Chemilumescence Assay Kit

The CD155:TIGIT [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed for screening and profiling molecules that block the binding between CD155 (cluster of differentiation 55) and TIGIT (T-cell immunoreceptor with Ig and ITIM domains). This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled TIGIT (amino acids 22-141), CD155 (amino acids 27-343), streptavidin-labeled HRP, and assay buffer for 100 reactions. A 96-well plate is coated with the human recombinant CD155 protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-TIGIT, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal is measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of CD155 to TIGIT.