CD155:CD96 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The CD155:CD96 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed for screening and profiling molecules that block the binding between CD155 (cluster of differentiation 155) and CD96 (cluster of differentiation 96). This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled CD96 (amino acids 22-503), CD155 (amino acids 27-343), streptavidin-labeled HRP, and assay buffer for 100 reactions. A 96-well plate is coated with CD155 protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-CD96, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added, and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of CD155 to CD96.