BLM Helicase Activity Assay Kit

The BLM Helicase Activity Assay Kit is a fluorogenic assay designed for screening and profiling of BLM (Bloom syndrome protein) antagonists/inhibitors by monitoring their inhibitory effects on BLM-catalyzed DNA unwinding. The BLM Helicase Activity Assay Kit comes in a convenient 96-well format, with enough purified recombinant BLM (amino acids 630-1300), ATP, DNA substrate, assay buffer, and additives for 100 reactions. The DNA substrate is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. BLM unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. BLM activity, therefore, results in a proportional increase in fluorescence.