beta-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit

Cayman's beta-HB (Ketone Body) Fluorometric Assay Kit provides a simple, reproducible, and sensitive tool for assaying beta-HB from plasma, serum, urine, tissue homogenates, and cell lysates. The method for beta-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme 3-hydroxybutyrate dehydrogenase.16 Concomitant with this oxidation, the cofactor NAD+ is reduced to NADH. NADH reacts with the fluorometric developer to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The fluorescence is directly proportional to the beta-HB concentration.Synonyms: beta-HB, 3-Hydroxybutyric Acid. Limit of Detection: 1.2 µM. Lower Limit of Quantification: 1.5 µM.