Alpha-Glucosidase Inhibitor Screening Kit

Alpha-glucosidase catalyzes the release of alpha-glucose from the non-reducing end of the substrate and promotes the absorption of glucose by the small intestine. Inhibition of alpha-glucosidase is an effective method for the treatment of non-insulin-dependent diabetes mellitus (NIDDM). The detection principle of this kit: alpha-Glucosidase catalyzes the substrate reaction to generate colored products, whose absorbance increases at 400 nm. The addition of an inhibitor suppresses the enzymatic activity of alpha-glucosidase, resulting in a reduced rate of absorbance increase. The inhibition rate can be calculated based on the absorbance difference.