Anti-IgG3 (human) Rabbit Monoclonal Antibody (Clone RM119)

Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response. It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum. IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each. The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively. IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization. IgG exists as four isotypes in humans, IgG1, IgG2, IgG3, and IgG4, each of which has a distinct effector function. IgG3 production is driven by bacterial- or viral-associated antigens, including HIV-1 and Staphylococcus antigens, and occurs early in the immune response following IgM class-switching. IgG3 binds to and neutralizes pathogens, as well as activates complement and opsonizes bacteria, leading to complement-dependent cytotoxicity (CDC) and ADCC, respectively. Serum IgG3 levels are increased in patients with primary biliary cirrhosis, Sjögren's syndrome, systemic sclerosis, or systemic lupus erythematosus (SLE). Cayman's IgG3 (human) Rabbit Monoclonal Antibody (Clone RM119) can be used for ELISA, immunocytochemistry (ICC), and immunohistochemistry (IHC) applications.Synonyms: Immunoglobulin G3. Immunogen: Human IgG3. Source: Rabbit. Clone Designation: RM119. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, ICC, IHC. Cross Reactivity: (+) Human IgG3 heavy chain, (-) Human IgG1, (-) human IgG2, (-) human IgG4, (-) human IgM, (-) human IgA, (-) human IgD, (-) human IgE, (-) Cynomolgus monkey, (-) goat, (-) mouse, (-) rat, (-) rhesus monkey.