Anti-Human GBA3 Antibody Pair

Product Description: Glucosylceramidase beta 3 (GBA3) is a human gene that encodes a cytosolic enzyme primarily involved in the hydrolysis of various glycosides, including beta-D-glucoside and beta-D-galactoside. This enzyme, classified under glycoside hydrolases, plays a significant role in detoxifying plant glycosides and has notable activity as a neutral glycosylceramidase, suggesting involvement in glucosylceramide metabolism outside lysosomal pathways. GBA3 is characterized as a polymorphic pseudogene due to mutations leading to a truncated protein, particularly the rs358231 variant, which introduces a premature stop codon and results in loss of function. This pseudogenization is thought to be influenced by dietary adaptations among different human populations, reflecting evolutionary changes where the enzyme's physiological role remains partially understood. The gene is located on chromosome 4 and has undergone multiple pseudogenization events across various mammalian lineages, indicating its complex evolutionary history. Protein Function: Neutral cytosolic beta-glycosidase with a broad substrate specificity that could play a role in the catabolism of glycosylceramides (PubMed:11389701, PubMed:11784319, PubMed:17595169, PubMed:20728381, PubMed:26724485, PubMed:33361282). Has a significant glucosylceramidase activity in vitro (PubMed:17595169, PubMed:26724485). However, that activity is relatively low and its significance in vivo is not clear (PubMed:17595169, PubMed:20728381, PubMed:26724485). Hydrolyzes galactosylceramides/GalCers, glucosylsphingosines/GlcSphs and galactosylsphingosines/GalSphs (PubMed:17595169). However, the in vivo relevance of these activities is unclear (PubMed:17595169). It can also hydrolyze a broad variety of dietary glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens in vitro and could therefore play a role in the metabolism of xenobiotics (PubMed:11784319). Possesses transxylosylase activity in vitro using xylosylated ceramides/XylCers (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) as xylosyl donors and cholesterol as acceptor (PubMed:33361282). Could also play a role in the catabolism of cytosolic sialyl free N-glycans (PubMed:26193330) [The Uniprot Consortium]