Anti-Human DIS3 Antibody Pair

Product Description: DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (DIS3) is a conserved hydrolytic enzyme that serves as a catalytic subunit of the RNA exosome complex, responsible for 3' to 5' degradation and processing of single-stranded RNA. It exhibits both processive exoribonuclease and endoribonuclease activities, with exonuclease function mediated by four conserved aspartic acid residues coordinating magnesium ions in its catalytic center, while the PIN domain provides endonucleolytic cleavage. DIS3 can unwind structured RNA substrates and its activity is modulated allosterically when part of the exosome complex. The protein is essential for RNA homeostasis and degradation of RNA intermediates in the nucleus and cytoplasm. Mutations in DIS3 are implicated in multiple myeloma, correlating with poorer patient survival, and often co-occur with chromosomal deletion del(13q). These mutations affect RNA processing and contribute to disease progression, underscoring DIS3's critical role in RNA metabolism and its impact on cancer pathogenesis. Protein Function: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities [The Uniprot Consortium]