Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »
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Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

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GAPD, Human GAPDH, Real Time PCR Primer Set
GAPD, Human GAPDH, Real Time PCR Primer Set

Item number: VHPS-3541

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: GAPD, GAPDH, CDABP0047, EC=1.2.1.12, EC=2.6.99.-, Peptidyl-cysteine S-nitrosylase GAPDH, Glyceraldehyde-3-phosphate...
Application: RNA quantification
43.00€ *
Review
E.coli Residual DNA Detection Kit
E.coli Residual DNA Detection Kit

Item number: ARG83091.100

E.coli Residual DNA Detection Kit is designed for the quantitative detection of E.coli host cell DNA in intermediates, semifinished products and finished products of various biological products. This kit adopts the principle of Taqman probe to quantitatively detect E.coli residual DNA in samples. The kit is a rapid,...
Application: qPCR
1,936.00€ *
Review
E.coli Residual Total RNA Detection Kit
E.coli Residual Total RNA Detection Kit

Item number: ARG83092.100

E.coli Residual Total RNA Detection Kit is designed for the quantitative detection of residual E.coli total RNA in various biological products to improve control quality of nucleic acid. This kit adopts the principle of the RT-PCR fluorescent probe, combining reverse transcription PCR technology and fluorescent...
Application: qPCR
2,480.00€ *
Review
Human Residual DNA Detection Kit
Human Residual DNA Detection Kit

Item number: ARG83095.100

Human Residual DNA Detection Kit is designed for the quantitative detection of the Human host cell DNA in intermediate, semifinished and finished products of various biological products. This kit adopts the principle of the Taqman probe to quantitatively detect Human residual DNA in samples. The kit is a rapid,...
Application: qPCR
1,936.00€ *
Review
Human Residual RNA Detection Kit
Human Residual RNA Detection Kit

Item number: ARG83096.100

Human Residual RNA Detection Kit is designed for the quantitative detection of residual Human total RNA in various biological products to improve control quality of nucleic acid. This kit adopts the principle of the RT-PCR fluorescent probe, combining reverse transcription PCR technology and fluorescent probe...
Application: qPCR
3,840.00€ *
Review
E1A and SV40LTA Residual DNA Detection Kit
E1A and SV40LTA Residual DNA Detection Kit

Item number: ARG83098.100

E1A and SV40LTA Residual DNA Detection Kit is designed for the rapid and specific detection of residual E1A and SV40LTA DNA derived from host cell in biological products. This kit adopts the fluorescent probe method and multiplex PCR method. The kit is a rapid, specific and reliable device, with the minimum...
Application: qPCR
2,864.00€ *
Review
Plasmid Residual DNA Detection Kit
Plasmid Residual DNA Detection Kit

Item number: ARG83100.100

Plasmid Residual DNA Detection Kit is designed for the quantitative detection of residual plasmid DNA in intermediates, semifinished products and finished products of various biological products. Plasmid DNA content in samples (e.g., lentivirus, adenovirus) is detected by analyzing the consensus sequence. This kit...
Application: qPCR
2,704.00€ *
Review
CHO Residual DNA Detection Kit
CHO Residual DNA Detection Kit

Item number: ARG83108.100

CHO Residual DNA Detection Kit is designed for the quantitative detection of residual Chinese hamster ovary DNA content in intermediates, semi-finished products and finished products of various biological products. CHO Residual DNA Detection Kit adopts the principle of the Taq-man probe to quantitatively detect CHO...
Application: qPCR
2,544.00€ *
Review
Vero Residual DNA Detection Kit
Vero Residual DNA Detection Kit

Item number: ARG83109.100

Vero Residual DNA Detection Kit is designed for the quantitative detection of residual Vero host cell DNA in intermediates, semi-finished products and finished products of various biological products. Vero Residual DNA Detection Kit adopts the principle of Taq-man probe to quantitatively detect residual Vero DNA in...
Application: qPCR
2,544.00€ *
Review
ACTB, Human actin, beta, Real Time PCR Primer Set
ACTB, Human actin, beta, Real Time PCR Primer Set

Item number: VHPS-110

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: ACTB, Beta-actin, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
Application: RNA quantification
43.00€ *
Review
Human Housekeeping Genes Primer Sets
Human Housekeeping Genes Primer Sets

Item number: HHK-1

Includes primer sets for ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, RPL13A, TBP and TFRC. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Application: RT-PCR, Gene expression analysis
138.00€ *
Review
TDO Primer Mix-qPCR Assay
TDO Primer Mix-qPCR Assay

Item number: BPS-71271

Primer mix for amplifying human TDO cDNA (1221 bp) using dye-based, real time, quantitative PCR (RT-qPCR).
Keywords: TO, TDO, TRPO, Tryptophanase, Tryptophan pyrrolase, Tryptophan oxygenase, Tryptamin 2,3-dioxygenase, Tryptophan...
Application: RT-qPCR, RT-PCR
Species reactivity: human
208.00€ *
Review
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