Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

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MCF-7 Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X SDS-PAGE Sample Buffer
MCF-7 Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X...

Item number: W09-000-360

Application: WB, Positive control
204.00€ *
Review
A431 Whole Cell Lysate Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X SDS-PAGE Sample Buffer
A431 Whole Cell Lysate Whole Cell Lysate, 1.0 mg/ml by...

Item number: W09-000-361

Application: WB, Positive control
204.00€ *
Review
EGF Stimulated A431 Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X SDS-PAGE Sample Buffer
EGF Stimulated A431 Whole Cell Lysate, 1.0 mg/ml by BCA...

Item number: W09-000-362

Application: WB, Positive control
258.00€ *
Review
Hep2 Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X SDS-PAGE Sample Buffer
Hep2 Whole Cell Lysate, 1.0 mg/ml by BCA assay, in 1X...

Item number: W09-000-363

Application: WB, Positive control
204.00€ *
Review
MCF-7 Whole Cell Lysate 1.0  mg/ml by Lowry assay in IX  SDS-Page Sample Buffer,  Hydrogen Peroxide
MCF-7 Whole Cell Lysate 1.0 mg/ml by Lowry assay in IX...

Item number: W09-000-366

Application: Positive Control for anti-ATM
302.00€ *
Review
HeLa Cell Nuclear Extract  in 1 x SDS-PAGE Sample Buffer
HeLa Cell Nuclear Extract in 1 x SDS-PAGE Sample Buffer

Item number: W09-001-367

Ready-to-use
Application: WB, Positive control
302.00€ *
Review
Raji Whole Cell Lysate
Raji Whole Cell Lysate

Item number: W09-001-368

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
204.00€ *
Review
HeLa Whole Cell Lysate TNFa Stimulated
HeLa Whole Cell Lysate TNFa Stimulated

Item number: W09-001-369

The cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were treated with 0.2 µg/ml TNF-alpha for 30 min. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium...
Application: WB
Host: Human
Species reactivity: human
302.00€ *
Review
A549 Whole Cell Lysate
A549 Whole Cell Lysate

Item number: W09-001-372

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB, Positive control
204.00€ *
Review
Human Derived U-2OS Whole Cell Lysate (Ready-to-Use)
Human Derived U-2OS Whole Cell Lysate (Ready-to-Use)

Item number: W09-001-374

Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB, Control
204.00€ *
Review
Human Foreskin Fibroblast whole cell lysate
Human Foreskin Fibroblast whole cell lysate

Item number: W09-001-375

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB
302.00€ *
Review
MDA-MD-435S Whole Cell Lysate (Ready-to-Use)
MDA-MD-435S Whole Cell Lysate (Ready-to-Use)

Item number: W09-001-A39

Ready-to-use whole cell lysate 1.0 mg/ml by Lowry assay in 1X SDS-PAGE sample buffer.
Application: WB, Positive control
204.00€ *
Review
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