The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LTA4H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LTA4H. Next,...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Leukotriene A4 Hydrolase (LTA4H) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of LTA4H in mouse serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.156-10ng/ml, Sensitivity: 0.051ng/ml, Storage and Stability: Desiccation...

Mouse LTA4H Recombinant Protein (RPES3299) is a highly pure recombinant protein developed by Assay Genie for use in a range of applications Protein function: Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the...

Protein function: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. [The UniProt Consortium]