The role of nitric oxide (NO) as a putative mediator of neuronal death can be understood best if NO is detected directly. 1,2-Diaminonaphthalene (DAN) is a fluorogenic probe that is used to detect nitric oxide (NO) productions in live cell and animals. The reaction product of DAN with NO is a triazole with blue...

Off-white to light brown solid. Soluble in DMSO, DMF or pyridine. The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN...

2,3-Diaminonaphthalene (DAN) is a fluorometric reagent for selenium and nitric oxide (NO). Upon binding to selenium, piazselenol is formed, which displays excitation/emission maxima of 365 and 525 nm, respectively. Upon binding to nitrite, the end product of the reaction between NO and oxygen in aqueous media,...