Products from PurSolutions

Use Actin Working Buffer supports the stability and activity of purified actin in vitro when supplemented with 0.2 mM ATP and 1 mM DTT. Composition: 5 mM Tris-HCl, 0.2 mM CaCl2, pH = 8.0.

Actin Polymer Buffer (10X) is comprised of 500 mM KCl and 20 mM MgCl2. Actin Polymer Buffer (10X) supports the polymerization of G-actin into F-actin in vitro. Polymerization Protocol: Dilute Ultra-Pure Actin (PUR-160101) to 0.4 mg/ml with Actin Working Buffer (PUR-000102, 5 mM Tris-HCl and 0.2 mM CaCl2, pH 8.0) supplemented with 0.2 mM ATP and 1 mM DTT. Incubate on ice for...

Source: Bovine Brain Material Labeled Tubulin-Biotin-XX is generated by reacting Biotin-XX NHS Ester with Cycled Tubulin (Cat. No. PUR-032005), thereby covalently linking the dye to random tubulin surface lysines. Cycled Tubulin is >99% pure and polymerization competent. These properties are maintained during the labeling process by reacting Cycled Tubulin with the reactive...

Source: Bovine Brain Material Labeled Tubulin-Alexa Fluor(R) 488 is generated by reacting Alexa Fluor(R) 488 NHS Ester (Thermo Fisher A20100) with Cycled Tubulin(TM) (Cat. No. PUR-032005), thereby covalently linking the dye to random tubulin surface lysines. Cycled Tubulin(TM) is >99% pure and polymerization competent. These properties are maintained during the labeling...

Source: Bovine Brain Material Labeled Tubulin-Alexa Fluor(R) 594 is generated by reacting Alexa Fluor(R) 594 NHS Ester (Thermo Fisher A20104) with Cycled Tubulin(TM) (Cat. No. PUR-032005), thereby covalently linking the dye to random tubulin surface lysines. Cycled Tubulin(TM) is >99% pure and polymerization competent. These properties are maintained during the labeling...

Source: Bovine Brain Material Labeled Tubulin-Alexa Fluor(R) 647 is generated by reacting Alexa Fluor(R) 647 NHS Ester (Thermo Fisher A20106) with Cycled Tubulin(TM) (Cat. No. PUR-032005), thereby covalently linking the dye to random tubulin surface lysines. Cycled Tubulin(TM) is >99% pure and polymerization competent. These properties are maintained during the labeling...

Source: Bovine Brain Material Lyophilized Tubulin was isolated by selective precipitation from bovine brain homogenate by an adaptation of the method of Andreu (2007) and lyophilized by an adaptation of the method of Dráberová et al. (2010). The resulting product is >99% pure and polymerization competent. Lyophilized Tubulin is supplied as a white powder with a...

Material Pure Actin was extracted from rabbit skeletal muscle using an optimized version of the method of Spudich and Watt (1971) and lyophilized by an adaptation of the method of Dráberová et al. (2010). The resulting product is >90% pure and >80% polymerization competent. Possible contaminants include a-actinin (100 kDa). For actin >99% pure, see our Ultra-Pure Actin...

Material Ultra-Pure Actin was extracted from rabbit skeletal muscle using an optimized version of the method of Spudich and Watt (1971) and lyophilized by an adaptation of the method of Dráberová et al. (2010). The resulting product is >99% pure and >90% polymerization competent. Ultra-Pure Actin is supplied as a white powder with an actin-to-powder weight ratio of 1:2. When...

Source: Bovine Brain Material Cycled Tubulin(TM) was isolated by cycling bovine brain homogenate through conditions that promote tubulin polymerization/depolymerization in high salt buffers by an adaptation of the method of Castoldi and Popov (2003). The resulting product is >99% pure and polymerization competent. Cycled Tubulin(TM) is supplied at 20 mg/ml as a frozen liquid...

Use Tubulin PEM Buffer supports the stability and activity of purified tubulin in vitro when supplemented with 1 mM each DTT and guanosine (GTP or GMPCPP). Composition: 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, pH = 6.8.