TrueBlot® Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG®) Epitope Tag

Item number Size Datasheet Manual SDS Delivery time Quantity Price
88-8887-383 1 kit - -

3 - 9 business days*

594.00€
 
This TrueBlot(R) Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG(R)) Epitope Tag... more
Product information "TrueBlot® Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG®) Epitope Tag"
This TrueBlot(R) Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG(R)) Epitope Tag allows for the detection of FLAG(R) -tagged recombinant protein present in cell lysates provided by the user. Mouse TrueBlot(R) ULTRA Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA. The FLAG(R) epitope is a small but highly immunogenic peptide DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) which allows fusion proteins to retain their original conformation and function. The hydrophilic character of this sequence increases the likelihood that it will be located on the surface of FLAG(R) -tagged recombinant proteins for reaction with anti-DYKDDDDK (FLAG(R)) antibodies. The anti-DYKDDDDK (FLAG(R)) antibody included in this Western blot kit detects FLAG(R) -tagged recombinant proteins fused with FLAG(R) tag either at the amino-terminus or carboxy-terminus of the protein. The TrueBlot(R) Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG(R)) Epitope Tag contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples containing the DYKDDDDK (FLAG(R)) Epitope Tag using TrueBlot second step immunoblotting reagents in conjunction with Rockland's Antibody for the detection of FLAG(TM) conjugated proteins (MOUSE) Monoclonal Antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot(R) ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality IP/WB data with Mouse IgG TrueBlot(R) ULTRA. Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
Keywords: TrueBlot(R), IP/WB, Anti-DYKDDDDK, Anti-FlagtTag
Supplier: Rockland Immunochemicals
Supplier-Nr: 88-8887-383

Properties

Application: IP, WB
Antibody Type: Monoclonal
Host: Mouse
Reactivity: Broad (Expected: Broad)
Format: Purified

Database Information

Handling & Safety

Storage: +4°C (do not freeze!)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "TrueBlot® Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG®) Epitope Tag"
Write a review
or to review a product.
Viewed