Goat TrueBlot® Set (with IP beads)

Goat TrueBlot® Set (with IP beads)
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Item number Size Datasheet Manual SDS Delivery time Quantity Price
88-1488-31 1 set - -

3 - 9 business days*

Goat IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-goat IgG immunoblotting... more
Product information "Goat TrueBlot® Set (with IP beads)"
Goat IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-goat IgG immunoblotting (second step) reagent. Goat IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Wester Blot data with Goat IgG TrueBlot®, simply substitute the conventional HRP anti-goat IgG blotting reagent with Goat IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Goat IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of goat IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Goat IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Goat IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.
Keywords: TrueBlot®, IP/WB
Supplier: Rockland Immunochemicals
Supplier-Nr: 88-1488-31


Application: IP, WB
Antibody Type: Monoclonal
Clone: eB270
Host: Mouse
Reactivity: Goat

Database Information

Handling & Safety

Storage: +4°C (do not freeze!)
Shipping: +4°C (International: +4°C)
Our products are for laboratory research use only: Not for administration to humans!
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