Calcein AM Cell Viability Assay Kit (Fluorometric)

Calcein AM Cell Viability Assay Kit (Fluorometric)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
G-BN00563.1000 1000 assays - - -

9 - 20 business days

371.00€
 
Calcein AM Cell Viability Assay Kit * Quantification of number of viable cells is an... more
Product information "Calcein AM Cell Viability Assay Kit (Fluorometric)"
Calcein AM Cell Viability Assay Kit * Quantification of number of viable cells is an indispensible tool in Cell Biology research. Assay Genie's Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min. * Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.* Figure: Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min. at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.* Key Information * Description * Product SKU* BN00563* Size* 1000 assays* Detection Method* Fluorescence (Ex/Em = 485/530 nm)* Application* Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients. * Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. * Assessment of physiological mediators and antibodies that affect cell growth.* Features & Benefits* Convenient & non-radioactive * High-throughput Highly-sensitive: detect as low as 50 viable cells in less than 30 min. * Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis* Kit Components* Calcein AM * Calcein Dilution Buffer*
Supplier: Assay Genie
Supplier-Nr: BN00563

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Our products are for laboratory research use only: Not for administration to humans!
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