ADCC Assay Kit

ADCC Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
G-BN00582.100 100 assays - - -

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343.00€
 
ADCC Assay Kit * Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis... more
Product information "ADCC Assay Kit"
ADCC Assay Kit * Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. Assay Genie's Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis.* Figure: Cytotoxicity Assay Kit: Jurkat cells (105 cells/ml) were grown in RPMI media supplemented with 10% FBS. Cells were treated with camptothecin (5 µM) overnight. Next day, cells were stained with CFSE and 7-AAD for 30 min. at 37°C. The graph (right side) displays the cytotoxic effect of the compound, illustrating apoptosis using CFSE and 7-AAD.* Key Information * Description * SKU* BN00582* Size* 100 assays* Measurement* Fluorescence (FL1 channel and FL3 channel)* Kit Overview* Direct method to measure cell-mediated cytotoxicity. Does not require cell lysis. Provides multi-parametric analysis.* Measurement of cell cytotoxicity in response to drug or toxin treatment * Quantification of the cytotoxic effect of immune effector cells on target cells * Assessment of physiological mediators and antibodies that affect cell cytotoxicity* Kit Contents* Cytotoxicity Assay Buffer * 7-AAD Staining Solution * CFSE Staining Solution* * Technical Manual
Supplier: Assay Genie
Supplier-Nr: BN00582

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