GenieClone Site-Directed Mutagenesis Kit v1

GenieClone Site-Directed Mutagenesis Kit v1
Item number Size Datasheet Manual SDS Delivery time Quantity Price
G-MORV0005.10 10 rxns - -

9 - 20 business days*

123.00€
G-MORV0005.25 25 rxns - -

9 - 20 business days*

258.00€
 
GenieClone Site-Directed Mutagenesis Kit v1 is designed for rapid site-directed mutagenesis of up... more
Product information "GenieClone Site-Directed Mutagenesis Kit v1"
GenieClone Site-Directed Mutagenesis Kit v1 is designed for rapid site-directed mutagenesis of up to 2 separate mutations in the same reaction in less than 3 hours. Based on GenieClone rapid cloning technology, this kit uses homologous recombination to replace the conventional annealing ring-forming reactions meaning much less template and a more flexible primer design strategy. With seamless splicing of the two PCR products by GenieClone technology, the kit can complete up to two separate mutations with a single amplification reaction. DpnI digested products of the specific amplicons can be directly added to the recombination reaction without purification. The highly optimized reaction buffer, fast protocol and superior site-directed mutagenesis efficiency make GenieClone Site-Directed Mutagenesis Kit v1 make an excellent choice for introducing 1 or 2 mutations at discontinuous sites simultaneously on the same plasmid. Key Features: * Introduce up to 2 separate mutations in the same reaction. * Site-directed mutagenesis can be performed on up to 2 discontinuous sites (more than 50 bp apart) simultaneously on the target plasmid. * Single kit for cloning and mutagenesis. * Includes Genie Fusion Ultra High-Fidelity DNA Polymerase providing high-fidelity PCR with extremely low mutation rates & excellent long fragment amplification for any plasmid amplification up to 20 kb as well as GC-rich templates. * Amplification is carried out exponentially and the template usage is extremely low, which is beneficial to the complete degradation of the original methylation template. * DpnI eliminates contamination of the original template and decreases background. * Amplified products can be directly used in the recombination reaction after DpnI treatment. * The reaction mixture can be used to directly transform chemically competent E.coli cells.
Supplier: Assay Genie
Supplier-Nr: MORV0005

Properties

Application: Up to 2 separate mutations

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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