Dienes Derivatization MaxSpec(R) Kit

This Dienes Derivatization MaxSpec(R) Kit is designed for the sensitive, accurate detection and quantitation of diene-containing compounds such as Vitamin D or Vitamin D metabolites. This is achieved through chemical derivatization utilizing a Derivatization Reagent based upon click chemistry. Included in this kit is a simple, rapid, and efficient protocol to extract and derivatize diene compounds from biological matrices. The resulting mixture reduces ion suppression and increases sensitivity with detection limits of 10-100 pM in most sample matrices. This extraction protocol is based upon protein precipitation and solid phase extraction (SPE) using the Dienes Derivatization MaxSpec(R) Kit and Waters HLB cartridges. After drying under vacuum, the extracted and concentrated diene compounds are directly dissolved in Derivatization Solution. The final mixture can be directly injected into an LC-MS/MS.