Buccutite(TM) Rapid APC Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per

Ex: 651 nm. Em: 662 nm. Soluble in: DMSO. APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm. AAT Bioquest offers this BuccutiteT rapid labeling kit to facilitate the APC conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. BuccutiteT APC Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC. The kit includes a preactivated APC and reaction buffer. The entire process only requires two simple mixings without further purification required. The conjugated antibody can be used in WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 25 ug of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC to facilitate the APC conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.