TCR Activator / PD-L1 Mammalian Expression Kit

Item number Size Datasheet Manual SDS Delivery time Quantity Price
BPS-60610 500 rxns - -

3 - 15 business days*

743.00€
 
The recombinant expression vectors are designed to express human engineered T cell receptor (TCR)... more
Product information "TCR Activator / PD-L1 Mammalian Expression Kit"
The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and human PD-L1 (GenBank Accession #NM_014143) in mammalian cells. The transfected cells can be used in conjunction with PD-1/NFAT Reporter/Jurkat T cells (BPS #60535) to study the interactions of PD-1 with PD-L1 ligand in a cellular context and screen for modulators of this signaling pathway In this assay, PD-1/NFAT Reporter/Jurkat T cells are used as effector cells, HEK293 cells overexpressing PD-L1 (or PD-L2) and an engineered T cell receptor (TCR) activator are used as target cells. When these two cells are co-cultivated, TCR complexes on effector cells are activated by TCR activator on target cells, resulting in expression of the NFAT luciferase reporter. However, PD1 and PD-L1 (or PD-L2) ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This inhibition can be specifically reversed by anti-PD1 or anti-PD-L1 antibodies. PD1/PD-L1 neutralizing antibodies block PD1:PD-L1 interaction and promote T cell activation, resulting in reactivation of the NFAT responsive luciferase reporter.
Keywords: B7H1, PD-L1, B7-H1, CD274, B7 homolog 1, PDCD1 ligand 1, Programmed death ligand 1, Programmed cell death 1 ligand 1
Supplier: BPS Bioscience
Supplier-Nr: 60610

Properties

Application: Activator / inhibitor screening, Biological activity characterization, Ligand interaction
Reactivity: Human

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -80°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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