Transferrin Protein (recombinant human)

Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD71 at 10 µg/ml (100 µl/well) can bind human Transferrin. The EC50 of human Transferrin is 5.6 ng/mL.2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.01-0.04 µg/mL. Protein Construction: Recombinant Human Transferrin is produced by our Mammalian expression system and the target gene encoding Val20-Pro698 is expressed with a 6His tag at the C-terminus. Sequence: Val20-Pro698. Fusion tag: C-6His Endotoxin: < 1.0 EU per µg as determined by the LAL method. Apparent Molecular Mass: 85 kDa. Protein function: Serotransferrin belongs to transferrin family, and contains 2 transferrin-like domains. The protein is a secreted protein, and expressed by the liver and secreted in plasma. Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Protein function: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. [The UniProt Consortium]