Spike RBD(L452R)(His Tag) (recombinant SARS-CoV-2)

Spike RBD(L452R)(His Tag) (recombinant SARS-CoV-2)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
E-PKSV030321.100 100 µg -

7 - 16 business days*

766.00€
E-PKSV030321.1 1 mg -

7 - 16 business days*

3,192.00€
 
Protein Construction: Recombinant SARS-CoV-2 S Protein RBD(L452R) is produced by Mammalian cells... more
Product information "Spike RBD(L452R)(His Tag) (recombinant SARS-CoV-2)"
Protein Construction: Recombinant SARS-CoV-2 S Protein RBD(L452R) is produced by Mammalian cells expression system and the target gene is expressed with C-His Tag Fusion tag: C-His Endotoxin: Please contact with the lab for this information Apparent Molecular Mass: 35kDa. Protein function: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. Protein function: [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32221306, PubMed:32075877, PubMed:32155444). Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell (PubMed:33082294, PubMed:33082293). This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2 (PubMed:33082293). The stalk domain of S contains three hinges, giving the head unexpected orientational freedom (PubMed:32817270). Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Can be alternatively processed by host furin (PubMed:32362314). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. [The UniProt Consortium]
Keywords: 2, Recombinant SARS-CoV-2 Spike RBD(L452R)(His Tag)
Supplier: Elabscience
Supplier-Nr: E-PKSV030321

Properties

Conjugate: No
Species reactivity: SARS-CoV-2
Format: Lyophilized

Handling & Safety

Storage: -80°C
Shipping: 4°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Spike RBD(L452R)(His Tag) (recombinant SARS-CoV-2)"
Write a review
or to review a product.
Viewed